{"id":39091,"date":"2018-01-19T12:08:20","date_gmt":"2018-01-19T06:38:20","guid":{"rendered":"https:\/\/new.editage.cn\/insights\/%e5%9b%bd%e5%ae%b6%e8%87%aa%e7%84%b6%e7%a7%91%e5%ad%a6%e5%9f%ba%e9%87%91%e7%94%b3%e6%8a%a5%ef%bc%9a%e7%b4%a7%e6%89%a3%e7%a7%91%e5%ad%a6%e9%97%ae%e9%a2%98%e7%9a%84%e7%a0%94%e7%a9%b6%e5%86%85%e5%ae%b9"},"modified":"2026-01-07T16:23:08","modified_gmt":"2026-01-07T10:53:08","slug":"guo-jia-zi-ran-ke-xue-ji-jin-shen-bao-jin-kou-ke-xue-wen-ti-de-yan-jiu-nei-rong-2012","status":"publish","type":"post","link":"https:\/\/www.editage.cn\/insights\/guo-jia-zi-ran-ke-xue-ji-jin-shen-bao-jin-kou-ke-xue-wen-ti-de-yan-jiu-nei-rong-2012\/","title":{"rendered":"\u56fd\u5bb6\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\u7533\u62a5\uff1a\u7d27\u6263\u79d1\u5b66\u95ee\u9898\u7684\u7814\u7a76\u5185\u5bb9"},"content":{"rendered":"<p>\u3010\u672c\u6587\u7ecf\u6388\u6743\u8f6c\u8f7d\u81ea<a href=\"https:\/\/blog.sciencenet.cn\/u\/tangxq\" rel=\"nofollow\" 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center;\"><strong>\u4e00\u3001\u4ec0\u4e48\u662f\u7814\u7a76\u5185\u5bb9\uff1f<\/strong><\/p>\n<p>\u7814\u7a76\u5185\u5bb9\u90e8\u5206\u662f\u56de\u7b54\u540c\u884c\u8bc4\u8bae\u4e13\u5bb6\u201c\u8fd9\u4e2a\u9879\u76ee\u7814\u7a76\u4ec0\u4e48\u201d\u7684\u95ee\u9898\uff0c\u4e5f\u5c31\u662f\u662f\u7533\u8bf7\u8005\u4e3a\u4e86\u8bba\u8bc1\u5047\u8bf4\u4ece\u4e0d\u540c\u65b9\u5411\uff08\u89d2\u5ea6\u3001\u5c42\u6b21\uff09\u8bbe\u8ba1\u7684\u7814\u7a76\u8ba1\u5212\u3002<\/p>\n<p>\u7814\u7a76\u5185\u5bb9\u662f\u4ee5\u79d1\u5b66\u95ee\u9898\u4e3a\u5bfc\u5411\uff0c\u7d27\u7d27\u56f4\u7ed5\u7814\u7a76\u5047\u8bf4\u800c\u7ec4\u7ec7\u8bbe\u8ba1\u7684\u5177\u4f53\u7684\u3001\u7ec6\u5316\u7684\u7814\u7a76\u6846\u67b6\uff1b\u662f\u7814\u7a76\u6d89\u53ca\u7684\u4e3b\u8981\u65b9\u9762\uff0c\u63cf\u8ff0\u4e3b\u8981\u5e72\u4ec0\u4e48\u4e8b\uff1b\u662f\u7814\u7a76\u76ee\u6807\u7684\u5177\u4f53\u4f53\u73b0\u4e0e\u5206\u89e3\u3002<\/p>\n<p style=\"text-align: center;\"><strong>\u4e8c\u3001\u8bbe\u8ba1\u7814\u7a76\u5185\u5bb9\u7684\u539f\u5219<\/strong><\/p>\n<p><strong>1.\u00a0<\/strong><strong>\u8981\u4ee5\u79d1\u5b66\u95ee\u9898\u4e3a\u5bfc\u5411\uff0c\u7d27\u7d27\u56f4\u7ed5\u7814\u7a76\u5047\u8bf4\u3002<\/strong>\u8981\u4ee5\u79d1\u5b66\u95ee\u9898\u4e3a\u5bfc\u5411\uff0c\u7d27\u7d27\u56f4\u7ed5\u7814\u7a76\u5047\u8bf4\u53bb\u7ec4\u7ec7\u8bbe\u8ba1\u53ef\u4ee5\u6700\u7ec8\u9a8c\u8bc1\u7814\u7a76\u5047\u8bf4\u6b63\u786e\u4e0e\u5426\u7684\u7814\u7a76\u8ba1\u5212\u548c\u7ebf\u8def\u3002\u5e94\u7740\u91cd\u8003\u8651\u7531\u79d1\u5b66\u95ee\u9898\u5ef6\u4f38\u51fa\u6765\u7684\u6700\u76f8\u5173\u8981\u7d20\uff0c\u7531\u79d1\u5b66\u95ee\u9898\u5f15\u7533\u51fa\u6765\u7684\u7814\u7a76\u5185\u5bb9\u4e0e\u79d1\u5b66\u95ee\u9898\u4e4b\u95f4\u662f\u6db5\u76d6\u5f0f\u7684\uff0c\u4e5f\u5c31\u662f\u8bf4\u7814\u7a76\u5185\u5bb9\u662f\u7531\u79d1\u5b66\u95ee\u9898\u5206\u89e3\u51fa\u6765\u7684\u591a\u4e2a\u5c0f\u7684\u79d1\u5b66\u95ee\u9898\u3002<\/p>\n<p><strong>2.\u00a0<\/strong><strong>\u7406\u987a\u7814\u7a76\u5185\u5bb9\u4e4b\u95f4\u7684\u5185\u5728\u903b\u8f91\u5173\u7cfb\u3002<\/strong>\u5728\u903b\u8f91\u4e0a\u8bb2\uff0c\u7814\u7a76\u5185\u5bb9\u4e4b\u95f4\u65e2\u8981\u76f8\u4e92\u72ec\u7acb\uff0c\u53c8\u8981\u76f8\u5173\u3002\u56e0\u6b64\uff0c\u7814\u7a76\u5185\u5bb9\u5207\u5fcc\u7b80\u5355\u7f57\u5217\uff0c\u7533\u8bf7\u8005\u8981\u6309\u7814\u7a76\u5185\u5bb9\u4e4b\u95f4\u5185\u5728\u7684\u903b\u8f91\u5173\u7cfb\uff0c\u5c42\u6b21\u5206\u660e\u5730\u5c55\u793a\uff0c\u8981\u4f53\u73b0\u51fa\u7533\u8bf7\u8005\u6e05\u6670\u7684\u7814\u7a76\u95ee\u9898\u7684\u601d\u8def\u3002\u8981\u63d0\u51fa\u7814\u7a76\u5185\u5bb9\u6240\u6d89\u53ca\u95ee\u9898\u7684\u5173\u952e\u70b9\u3002\u4e0d\u4ec5\u5199\u201c\u505a\u4ec0\u4e48\u201d\uff0c\u66f4\u5e94\u8be5\u5199\u51fa\u201c\u600e\u4e48\u7814\u7a76\u201d\u53ca\u201c\u5177\u4f53\u7684\u7814\u7a76\u601d\u8def\u201d\u3002<\/p>\n<p 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style=\"text-align: center;\"><strong>\u56db\u3001 <\/strong><strong>\u64b0\u5199\u7814\u7a76\u5185\u5bb9\u65f6\u5e38\u89c1\u7684\u95ee\u9898<\/strong><\/p>\n<p><strong>1.\u00a0<\/strong><strong>\u6ca1\u6709\u7d27\u6263\u79d1\u5b66\u95ee\u9898<\/strong><\/p>\n<p>\u79d1\u5b66\u95ee\u9898\u662f\u9879\u76ee\u7684\u6838\u5fc3\uff0c\u7814\u7a76\u9879\u76ee\u5b9e\u65bd\u7684\u8fc7\u7a0b\u5c31\u662f\u5bf9\u79d1\u5b66\u95ee\u9898\u505a\u51fa\u89e3\u7b54\u7684\u8fc7\u7a0b\uff0c\u56e0\u6b64\u7814\u7a76\u5185\u5bb9\u8981\u56f4\u7ed5\u62df\u89e3\u51b3\u7684\u5173\u952e\u79d1\u5b66\u95ee\u9898\u6765\u8bbe\u8ba1\uff0c\u4e0d\u540c\u7684\u5b9e\u9a8c\u7814\u7a76\u90fd\u56f4\u7ed5\u4e00\u70b9\uff0c\u671d\u79d1\u5b66\u95ee\u9898\u7684\u66f4\u6df1\u5c42\u9762\u5c55\u5f00\u3002<\/p>\n<p><strong>2.\u00a0<\/strong><strong>\u7814\u7a76\u5185\u5bb9\u4e0e\u7814\u7a76\u65b9\u6848\u76f8\u4e92\u8131\u8282<\/strong><\/p>\n<p>\u5982\u679c\u8bf4\u7814\u7a76\u5185\u5bb9\u5b8c\u6210\u8fc7\u7a0b\u662f\u5bf9\u79d1\u5b66\u95ee\u9898\u505a\u51fa\u89e3\u7b54\u7684\u8fc7\u7a0b\uff0c\u90a3\u4e48\u7814\u7a76\u65b9\u6848\u5b9e\u65bd\u8fc7\u7a0b\u5c31\u662f\u7814\u7a76\u5185\u5bb9\u5b8c\u6210\u8fc7\u7a0b\u3002\u7533\u8bf7\u4e66\u4e2d\u5e38\u89c1\u7684\u4e00\u4e2a\u8d25\u7b14\u662f\u7814\u7a76\u5185\u5bb9\u4e0e\u7814\u7a76\u65b9\u6848\u4e4b\u95f4\u76f8\u4e92\u8131\u8282\uff0c\u7814\u7a76\u5185\u5bb9\u8981\u56de\u7b54\u7684\u95ee\u9898\u5728\u7814\u7a76\u65b9\u6848\u4e2d\u6ca1\u6709\u53ef\u5b9e\u65bd\u65b9\u6848\uff0c\u6216\u8005\u5b9e\u65bd\u7814\u7a76\u65b9\u6848\u540e\u4f3c\u4e4e\u4e0d\u80fd\u56de\u5e94\u7814\u7a76\u5185\u5bb9\u3002\u56e0\u6b64\u8981\u7279\u522b\u6ce8\u610f\u7814\u7a76\u5185\u5bb9\u4e0e\u7814\u7a76\u65b9\u6848\u7684\u4e00\u81f4\u6027\u539f\u5219\u3002<\/p>\n<p><strong>3.\u00a0<\/strong><strong>\u7814\u7a76\u5185\u5bb9\u4e0d\u547c\u5e94\u9009\u9898<\/strong><\/p>\n<p>\u7814\u7a76\u5185\u5bb9\u8981\u56f4\u7ed5\u7814\u7a76\u5047\u8bf4\u548c\u7814\u7a76\u76ee\u6807\u53bb\u8bbe\u8ba1\u548c\u9009\u62e9\uff0c\u8981\u547c\u5e94\u9009\u9898\u3002\u7814\u7a76\u5185\u5bb9\u4e0d\u547c\u5e94\u9009\u9898\uff0c\u5f80\u5f80\u662f\u7531\u4e8e\u7814\u7a76\u5185\u5bb9\u504f\u79bb\u4e3b\u9898\uff0c\u6216\u8005\u8bbe\u7f6e\u7684\u7814\u7a76\u5185\u5bb9\u8f83\u591a\uff0c\u800c\u4e14\u7814\u7a76\u5185\u5bb9\u4e4b\u95f4\u7684\u5173\u7cfb\u53c8\u4e0d\u5341\u5206\u7d27\u5bc6\u3002<\/p>\n<p><strong>4.\u00a0<\/strong><strong>\u7814\u7a76\u5185\u5bb9\u201c<\/strong><strong>\u5927\u201d<\/strong><strong>\u800c\u201c<\/strong><strong>\u7a7a\u201d<\/strong><strong>\u3001\u91cd\u70b9\u4e0d\u7a81\u51fa<\/strong><\/p>\n<p>\u7814\u7a76\u5185\u5bb9\u8981\u80fd\u591f\u987a\u5229\u5b9e\u65bd\uff0c\u5982\u679c\u5927\u800c\u7a7a\uff0c\u52bf\u5fc5\u5bfc\u81f4\u7814\u7a76\u5185\u5bb9\u592a\u6563\u3001\u6df1\u5ea6\u4e0d\u591f\u3001\u7814\u7a76\u76ee\u6807\u4e0d\u96c6\u4e2d\u3002\u6709\u4e9b\u4eba\u4f3c\u4e4e\u8ba4\u4e3a\uff0c\u8ba1\u5212\u5185\u5bb9\u5b81\u53ef\u591a\u4e00\u4e9b\uff0c\u4e5f\u4e0d\u80fd\u592a\u5c11\uff0c\u5e38\u6784\u601d\u51fa\u9700\u8981\u51e0\u7bc7\u535a\u58eb\u8bba\u6587\u624d\u80fd\u5b8c\u6210\u7684\u9879\u76ee\uff0c\u7ecf\u8d39\u8d44\u52a9\u673a\u6784\u5374\u4e0d\u53ef\u80fd\u63a5\u53d7\u5982\u6b64\u201c\u4e0d\u7740\u8fb9\u201d\u7684\u7533\u8bf7\u3002\u8ba1\u5212\u5185\u5bb9\u8fc7\u591a\u4f1a\u8ba9\u4eba\u5bf9\u4f60\u7684\u5224\u65ad\u80fd\u529b\u4ea7\u751f\u6000\u7591\uff0c\u5e76\u4e14\u8bc4\u5ba1\u4eba\u4e5f\u65e0\u6cd5\u5f97\u77e5\u4f60\u662f\u5982\u4f55\u533a\u5206\u7814\u7a76\u5185\u5bb9\u7684\u91cd\u70b9\u7684\u3002<\/p>\n<p><strong>5.\u00a0<\/strong><strong>\u7814\u7a76\u5185\u5bb9\u8fc7\u4e8e\u7b80\u5355<\/strong><\/p>\n<p>\u4f8b\u5982\uff0c\u201c\u4e2d\u533b\u836f\u5e72\u9884\u5728\u539f\u53d1\u6027\u809d\u764c\u7684\u57fa\u7840\u4e0e\u5e94\u7528\u7814\u7a76\u201d\uff0c\u9898\u76ee\u5f88\u5927\uff0c\u800c\u7814\u7a76\u5185\u5bb9\u4ec5\u662f\u6536\u96c6\u539f\u53d1\u6027\u809d\u764c\u75c5\u4eba\u7684\u4e34\u5e8a\u8d44\u6599\u3001\u75c5\u7406\u8d44\u6599\u3001\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66\u67d3\u8272\u5b9e\u9a8c\u7ed3\u679c\uff0c\u7535\u955c\u89c2\u5bdf\u7ed3\u679c\uff0c\u7136\u540e\u662f\u4e00\u7ec4\u7ed3\u5408\u4e2d\u533b\u8fa8\u8bc1\u8bba\u6cbb\u7684\u6240\u8c13\u201c\u4e2d\u533b\u836f\u5e72\u9884\u7ec4\u201d\uff0c\u6839\u672c\u672a\u4f53\u73b0\u201c\u57fa\u7840\u4e0e\u5e94\u7528\u7814\u7a76\u201d\u5185\u5bb9\u3002\u6709\u7684\u9879\u76ee\u7814\u7a76\u5185\u5bb9\u53ea\u7b80\u5355\u5730\u7528\u51e0\u53e5\u533b\u5b66\u672f\u8bed\uff0c\u65e2\u4e0d\u662f\u5c11\u800c\u7cbe\u7684\u9ad8\u5ea6\u6982\u62ec\uff0c\u4e5f\u4e0d\u662f\u5168\u9762\u7cfb\u7edf\u5730\u8bba\u8ff0\uff0c\u8fd9\u6837\u4f1a\u4f7f\u8bc4\u9605\u4eba\u65e0\u6cd5\u5145\u5206\u4e86\u89e3\u9009\u9898\u7684\u6df1\u5c42\u6b21\u5185\u6db5\u3002<\/p>\n<p><strong>6.\u00a0<\/strong><strong>\u628a\u7814\u7a76\u65b9\u6cd5\u5f53\u7814\u7a76\u5185\u5bb9\u6765\u5199<\/strong><\/p>\n<p>\u7814\u7a76\u5185\u5bb9\u662f\u7814\u7a76\u76ee\u6807\u7684\u5177\u4f53\u4f53\u73b0\u4e0e\u5206\u89e3\uff0c\u662f\u7814\u7a76\u6d89\u53ca\u7684\u4e3b\u8981\u65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A \u836f\u5bf9 B \u57fa\u56e0\u8868\u8fbe\u7684\u8c03\u63a7\u4f5c\u7528\uff0c\u8fd9\u662f\u4e00\u4e2a\u8f83\u597d\u7684\u7814\u7a76\u5185\u5bb9\uff0c\u5982\u679c\u5199\u6210\u201cWestern blot \u68c0\u6d4b B \u57fa\u56e0\u7684\u8868\u8fbe\u201d\uff0c\u8fd9\u5c31\u8ba9\u540c\u884c\u65e0\u6cd5\u77e5\u9053\u4f60\u7684\u7814\u7a76\u5207\u5165\u70b9\u662f\u4ec0\u4e48\uff0c\u65e0\u6cd5\u660e\u767d\u4f60\u8981\u63a2\u8ba8\u4e00\u4e2a\u4ec0\u4e48\u6837\u7684\u79d1\u5b66\u95ee\u9898\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u672c\u6587\u7ecf\u6388\u6743\u8f6c\u8f7d\u81ea\u5510\u5c0f\u537f\u79d1\u5b66\u7f51\u535a\u5ba2\uff0c\u8bf7\u70b9\u6b64\u67e5\u770b\u539f\u6587\u3011 \u4e00\u3001\u4ec0\u4e48\u662f\u7814\u7a76\u5185\u5bb9\uff1f \u7814\u7a76\u5185\u5bb9\u90e8\u5206\u662f\u56de\u7b54\u540c\u884c\u8bc4\u8bae\u4e13\u5bb6\u201c\u8fd9 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