You are here

Researchers develop a new techinque to observe live cells in action

Editage Insights | 2015年9月2日 | 2,723 浏览次数
Researchers develop a new approach to observe live cells in action

Eric Betzig of Janelia Research Campus in Ashburn, Virginia, who won the 2014 Nobel Prize in Chemistry for the development of super-resolved fluorescence microscopy, and his colleagues have applied their techniques to watching live cells in action and generated images and videos of protein movement and interactions as the cells internalize molecules. Betzig and his team improved the spatial resolution of structured illumination microscopy (SIM) that conventionally generates images by switching on the fluorescent labels that researchers use to tag specific proteins and then deactivating most of them. The tags in the darkest regions continue to fluoresce, which sharpens the image. However, the time it takes to switch the tags on and off has made it difficult to apply the technique to live cells. Therefore, Betzig and his colleagues used an approach called patterned photoactivation nonlinear SIM that involves turning on only some fluorescent tags at a time. Using this technique, they could observe proteins breaking down and changing shape. They were also able to see the dynamics of cell-surface pits called caveolae. An additional advantage of the new approach is that it enables generating images at a faster pace than before; the team was able to capture 25 snapshots in about one-third of a second.

Read more in The Scientist.   


《意得辑专家视点》深信知识需要开放给所有大众并传播,因此我们鼓励读者重复发表我们的内容,重复发表形式可为在线或印刷。我们采用知识共享(Creative Commons license),只要您遵守以下事项,即可免费重复发表我们的内容:
  • 作者信息:请尊重我们的作者,他们花费了时间精力为您撰写这些有价值的内容,重复发表时加注作者信息。
  • 意得辑专家视点:必须注明文章出自《意得辑专家视点》。
  • 表达您的情意:您可以加句“前往《意得辑专家视点》阅读全文”之类的话,啊,还有,别忘了加上文章链接。
  • 重复使用图片:要使用某些文章的图片必须事先取得许可,并加注图片原始出处。
  • 镶嵌代码:要重复使用这篇文章最简单的方式就是将下面的代码复制贴上您的页面!